|
| Status |
Public on Sep 23, 2015 |
| Title |
Adeno non Inflating d100_replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Organ (spleen) CD8+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: spleen
cell type: CD8+ T cells
Stage: 100 days post infection
|
| Treatment protocol |
MCMV was propagated and titrated on NIH 3T3 cells (ECACC, UK), stored at -80°C and injected intravenously at a dose of 2x106 pfu; Recombinant adenovirus expressing the βgal protein under the control of the human CMV promoter (Ad-LacZ) lacking E1 and E3 genes was used (Krebs et al., 2005). Ad-LacZ was propagated on permissive HER-911 cells and purified with the Vivapure AdenoPack 20 (Sartorius, Stedim biotech, 13781 Aubagne Cedex, France) according to manufacturer’s specifications. Virus titer was determined in a cytopathic effect assay (Krebs et al., 2005). Ad-LacZ was stored at -80°C in PBS and injected intravenously at a dose of 2x109 pfu.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from sorted cells according to manufactures instructions using the mirVana Kit (Ambion). RNA integrity was checked using Agilent 2100 Bioanalyzer, samples with a RIN value of 8 or more were used for amplification
|
| Label |
Biotin
|
| Label protocol |
cDNA was synthesized and cRNA made using Illumina Total Prep RNA amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Samples were hybridized following protocols for Illumina MouseWG-6 v2.0 beadchip.
|
| Scan protocol |
Illumina GenomeStudio software (v1.0.6) was used to process scanner images and produce raw microarray outputs
|
| Description |
S8_I8V_100
replicate 3
5785978001_B.idat
|
| Data processing |
Probe intensity levels were generated from Illumina GenomeStudio software outputs and normalised using R/Bioconductor tools (vsn normalisation)
|
| |
|
| Submission date |
Sep 22, 2015 |
| Last update date |
Sep 23, 2015 |
| Contact name |
Timothy Hinks |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Oxford
|
| Department |
NDM Experimental Medicine - New College
|
| Street address |
John Radcliffe Hospital, Headley Way, Oxford
|
| City |
Oxford |
| State/province |
United Kingdom |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE73314 |
Adenoviral vector vaccination induces a conserved program of CD8+ T cell memory differentiation in mouse and man |
|
