|
| Status |
Public on Sep 22, 2015 |
| Title |
CTKD_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cortical neurons transduced with control lentivirus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: cortical neuron
genotype: Dicerflox/flox
treatment: control
|
| Treatment protocol |
Lentivirus (MOI=5) was introduced to neurons after they had attached on the dish
|
| Growth protocol |
500,000 neurons were seeded on 12-well poly-L-lysine-coated tissue culture dishes and cultured in Neurobasal media with B27 supplement for two weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA were prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion Inc.) from 500ng of total RNA.
|
| |
|
| Hybridization protocol |
1.5ug of biotin-labeled cRNA were hybridized on the Illumina Mouse WG-6 version 2 beadchip according to Manufacturer’s instruction (Illumina, Inc., San Diego, CA)
|
| Scan protocol |
Beadchips were washed and stained according to Manufacturer’s instruction and scanned using Illumina BeadArray Reader (Illumina, Inc., San Diego, CA)
|
| Description |
SAMPLE 1
|
| Data processing |
The data were normalized using quantile normalization with R according to 'Analyzing An Illumina Microarray Dataset Using R and Bioconductor' by Giovanni Coppola, August 2010
|
| |
|
| Submission date |
Sep 21, 2015 |
| Last update date |
Sep 22, 2015 |
| Contact name |
Vivian Y Poon |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke-NUS Graduate Medical School
|
| Street address |
8 College Road
|
| City |
Singapore |
| State/province |
SINGAPORE |
| ZIP/Postal code |
169857 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE73275 |
The effect of knocking down miR-27b on DIV14 mouse cortical neurons |
|
