|
| Status |
Public on Jul 28, 2016 |
| Title |
Liver_KC_Irf8_ko3 |
| Sample type |
RNA |
| |
|
| Source name |
adult Kupffer cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
dev.stage: adult
celltype: macrophages
genotype: Irf8 knockout
|
| Treatment protocol |
samples from liver, kidney and skin were treated with collagenase. Skin and Liver was additionaly treated with dispase and DNase for digesting the tissue. Brain cells (microglia) were isolated by a percoll-gradient.
|
| Growth protocol |
isolated cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was perfomed using Arcturus PicoPure isolation kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Scan protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Description |
adult Kupffer cells Irf8KO rep3
|
| Data processing |
Background correction and normalization was performed using the RMA-Sketch implementation in Affymetrix Expression Console
|
| |
|
| Submission date |
Sep 17, 2015 |
| Last update date |
Jul 28, 2016 |
| Contact name |
Ori Staszewski |
| E-mail(s) |
[email protected]
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Institute of Neuropathology
|
| Street address |
Breisacher Str. 64
|
| City |
Freiburg |
| ZIP/Postal code |
D-79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE73125 |
Transcriptome-based profiling reveals a macrophage pedigree and identifies Irf8 as pivotal for macrophage homeostasis and function |
|
