|
| Status |
Public on May 12, 2007 |
| Title |
Dioxin treated MCF7 breast cancer cells [HG-U133B] Exp 1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 human breast cancer cells, dioxin-treated
|
| Organism |
Homo sapiens |
| Characteristics |
16 hr dioxin treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Enzo Biotin labeling
|
| |
|
| Hybridization protocol |
15ug of CRNA was fragmented, fragmented product was checked on gel(average size 100nt), Hybridization cocktail added and hybed on HG_U133B arrays. Washed with non-stringent wash buffer (A) and stained with SAPE solution.
|
| Scan protocol |
HP Genechip Scanner (fixed pixel and wavelength)
|
| Description |
MCF7 human breast cancer cells treated with 100 nM of dioxin to quantify RNA differences in a 2 platform, three replicate experiment
|
| Data processing |
Probe array type- HG_U133 B. Parameters - defaults. Normalization - all probe sets. Scaling - all probe sets (Target signal - 2500)
|
| |
|
| Submission date |
May 08, 2007 |
| Last update date |
May 11, 2007 |
| Contact name |
Oliver Hankinson |
| E-mail(s) |
[email protected]
|
| Phone |
(310) 825-2936
|
| Fax |
(310) 794-9272
|
| Organization name |
UCLA
|
| Department |
Pathology and Laboratory Medicine
|
| Lab |
CHS-Hankinson
|
| Street address |
PO Box 951732
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1732 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE7765 |
Dioxin-induced gene expression changes in MCF-7 human breast cancer cells |
|
