|
| Status |
Public on Nov 01, 2015 |
| Title |
HN |
| Sample type |
SRA |
| |
|
| Source name |
Serratia, low nitrogen
|
| Organism |
Serratia sp. ZM |
| Characteristics |
strain/background: ZM
genotype/variation: wild type
growth condition: high nitrogen
growth time: 10 hours
|
| Growth protocol |
Growth in low nitrogen (2mM (NH4)2SO4) or high nitrogen (10mM (NH4)2SO4) conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 10 hours, cells were processed for total RNA extraction using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Serratia sp. ZM
|
| Data processing |
Primary sequencing data was produced by Illumina HiSeqTM 2000.
Sequenced reads were subjected to quality control, then mapped to Serratia sp. SCBI whole genome with SOAPaligner/SOAP2.
The gene expression level is calculated by using RPKM method (Reads per kilobase transcriptome per million) mapped reads.
Genome_build: Serratia sp. SCBI (CP003424.1)
Supplementary_files_format_and_content: Files include RPKM values for each Sample.
|
| |
|
| Submission date |
Sep 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Pei Hai yan |
| E-mail(s) |
[email protected]
|
| Phone |
18317052938
|
| Organization name |
East China University of Science and Technology
|
| Street address |
No. 130, meilong road, Shanghai
|
| City |
shanghai |
| ZIP/Postal code |
200237 |
| Country |
China |
| |
|
| Platform ID |
GPL20923 |
| Series (1) |
| GSE72965 |
The transcriptome of Serratia sp. ZM under different concentrations of (NH4)2SO4 |
|
| Relations |
| BioSample |
SAMN04054375 |
| SRA |
SRX1225797 |
