Total RNA was isolated from frozen liver tissues (3 livers pooled samples for each condition, in duplicates). Perfect Pure RNA Tissue Kit (5Prime, Darmstadt, Germany) following the manufacturer’s instructions
Label
biotin
Label protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Hybridization protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Description
gene expression data of SFA diet pups liver C57Bl6/J mice, 4–5 weeks old, were used. Female mice were divided in three groups that received one of three isocaloric diets, containing 6g% commercial oil of different origins as follows: soybean in control diet, coconut in SFA and flaxseed in ALA. Oleic acid in RD (42%), ALA in ALA diet (31.6%) and the saturated fatty acids in SFA diet (48.51%) were the predominant FA in the maternal diets. Males received regular chow diet except during the mating period during which they shared their female mates' diets. At all stages, the animals had free access to food and water and were kept in ventilated rooms under a light/dark cycle of 12h/12h. Within a day after birth, offspring's livers were excised and snap frozen in liquid nitrogen for further use.
Data processing
Probe-set analyses was performed. RMA normalization and summarization by mean, all were done by Partek Genomics suite (version 6.6)
