|
| Status |
Public on May 05, 2007 |
| Title |
Polyamide1_2 |
| Sample type |
RNA |
| |
|
| Source name |
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cultured LNCaP cells
refer to ATTC
|
| Treatment protocol |
After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
|
| Growth protocol |
LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| Scan protocol |
Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| Description |
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Data processing |
Resolver (Rosetta Biosoftware, Seattle)
|
| |
|
| Submission date |
May 04, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Peter Dervan |
| Organization name |
California Institute of Technology
|
| Street address |
1200 East California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7708 |
Suppression of androgen receptor mediated gene expression by a sequence-specific DNA binding polyamide |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
