|
| Status |
Public on Aug 17, 2016 |
| Title |
Matn4-/- 1 |
| Sample type |
RNA |
| |
|
| Source name |
Matn4-/-_hematopoietic stem cells, 4 weeks post transplant
|
| Organism |
Mus musculus |
| Characteristics |
cell type: hematopoietic stem cells
gender: female
strain: C57/BL6
age: 12 weeks
genotype: Matrilin-4-/-
|
| Treatment protocol |
Recipient mice (CD45.1) were irradiated with 2x500Rad and transplanted with a 1:1 mixture of wt and Matn4-/- bone marrow (3x10^6 cells).
|
| Growth protocol |
wt (CD45.1.2) and Matn4-/- (CD45.2) HSCs (LSKCD150+CD48-) sorted by flow cytometry
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated using the Arturus RNA isolation kit.
|
| Label |
biotin
|
| Label protocol |
The NuGEN Ovation Pico WTA System (linear isothermal DNA amplification process) is used to generate single-stranded cDNA from small input amounts of 2.5ng RNA. Labeled cRNA were prepared according to Nugene Encore BiotinIL Module.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridiation protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Sep 08, 2015 |
| Last update date |
Aug 17, 2016 |
| Contact name |
Hannah Uckelmann |
| E-mail(s) |
[email protected]
|
| Phone |
+49 6221423916
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Department |
Stem Cells and Cancer
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17543 |
| Series (2) |
| GSE72813 |
Gene expression analysis of transplanted wt and Matn4-/- hematopoietic stem cells (HSC) |
| GSE72814 |
Extracellular matrix protein Matrilin-4 regulates HSC stress response |
|
