|
| Status |
Public on Apr 30, 2016 |
| Title |
H5V cells incubated with LD-Exo, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
H5V cells treated with LD-Exo
|
| Organism |
Mus musculus |
| Characteristics |
cell type: heart endothelial cell
|
| Treatment protocol |
After 24 h, cells were incubated with LD-Exo or HD-Exo, at a concentration of 15 µg/ml or PBS for 24 hours.
|
| Growth protocol |
H5V cells were seeded in 24 well plates at a density of 60,000 cells per well in DMEM (Life Technologies) supplemented with 10% HI FCS depleted of serum Extracellular vesicles (EVs) and incubated for 24h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNeasy Mini Kit (Qiagen, Hilden, Germany) .
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA was hybridised on to Mouse Gene ST 2.1 Array plates.
|
| |
|
| Hybridization protocol |
RNA was hybridised on to Mouse Gene ST 2.1 Array plates.
|
| Scan protocol |
Image files from scanning (.dat and .cel files) were generated with Affymetrix Expression Console package.
|
| Description |
mRNA expression data from H5V cells incubated with LD-Exo
|
| Data processing |
The arrays were analysed using Median Polish, RMA background correction and Sketch-Quantile normalisation (RMA-Sketch in Expression Console Software). Only the signals from the probe sets in the main category were used. If a probe set signal was below 20 in all the groups, it was removed.
|
| |
|
| Submission date |
Aug 25, 2015 |
| Last update date |
Apr 30, 2016 |
| Contact name |
Pieter Vader |
| Organization name |
University of Oxford
|
| Department |
Department of Physiology, Anatomy and Genetics
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE72351 |
Cells release subpopulations of exosomes with distinct molecular and functional properties |
|
