Genomic DNA was extracted from peripheral blood using a homemade DNA extraction kit based on the salting-out method, and dissolved in low TE (1mM Tris-Hcl pH=8, 0.1mM EDTA). DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
Label
biotin
Label protocol
As per manufacturer (Affymetrix)
Hybridization protocol
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 7G.
Description
Hybridized to 250K_Nsp
Data processing
The array image was acquired using Affymetrix GeneChip® Command Console® Software (AGCC) . Copy number values for individual SNPs were extracted and converted from CEL files into signal intensities using the Genotyping Consule software.
