|
| Status |
Public on Oct 24, 2007 |
| Title |
Control, t=120 min, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
yeast, Control, t=120 min, replicate 2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain BY4743
Mid-exponental phase
|
| Treatment protocol |
In mid-exponential phase (OD600 ~ 1.5), three replicate cultures were treated with 0.19 mM CHP, while three untreated cultures were used as controls.
|
| Growth protocol |
Yeast cultures were grown in controlled batch conditions, in 1 L fermentors at 30C, pH 6.0, 500 rpm, and dO2> 80%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with a procedure modified from the hot phenol protocol, as described (Martins, A. et al (2007) Yeast 24, 181-188)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast S98 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 640.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G Scanner.
|
| Description |
Control (untreated culture), collected at t=120 min (after adding CHP to treated cultures), biological replicate 2
|
| Data processing |
The data were normalized with RMA.
|
| |
|
| Submission date |
Apr 26, 2007 |
| Last update date |
Jul 10, 2007 |
| Contact name |
Vladimir Shulaev |
| E-mail(s) |
[email protected]
|
| Organization name |
Virginia Bioinformatics Institute
|
| Street address |
Virginia Bioinformatics Inst., Virginia Tech, Washington st.
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE7645 |
Expression data for Saccharomyces cerevisiae oxidative stress response |
|
