|
| Status |
Public on Aug 13, 2015 |
| Title |
HCT116 p53 null control |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 p53 null control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colon cancer
cell line: HCT116 p53 null
|
| Treatment protocol |
HCT116 &HCT116 p53null with or without Ectoposide
|
| Growth protocol |
mouse embryonic fibroblast & p53-/-mouse embryonic fibroblast (normal culture condition), HCT116 &HCT116 p53null with or without Ectoposide
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIZOL reagent according to manufacturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Cy3- cRNA were prepared with the Illumina TotalPrep™ RNA amplification kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Arrays were scanned on an Illumina BeadArray Reader following the standard Illumina scanning protocol.
|
| Description |
rep1
|
| Data processing |
The data were background subtracted and normalized using Illumina GenomeStudio
|
| |
|
| Submission date |
Aug 12, 2015 |
| Last update date |
Feb 02, 2016 |
| Contact name |
Dong-Hoon Jin |
| E-mail(s) |
[email protected]
|
| Phone |
82-2-3010-2643
|
| Organization name |
Asan Medical Center
|
| Street address |
88 Olympicro-43gil, Songpa-gu
|
| City |
Seoul |
| ZIP/Postal code |
138-736 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE71980 |
Foxp3 is a key downstream regulator of p53-mediated cellular senescence |
|
