|
| Status |
Public on Jun 13, 2016 |
| Title |
Rac1 biological replicate #1 |
| Sample type |
RNA |
| |
|
| Source name |
Wholeskin Rac1 pup D7
|
| Organism |
Mus musculus |
| Characteristics |
strain: CBA/CaJ
age: Day7
tissue: Whole skin
genotype/variation: overexpressing epidermal specific (K14) activated mutant (V12) Rac1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a Qiagen RNA miniprep kit. RNA concentration was determined with spectrophotometric analysis; purity analyzed with 260:280 absorbance ratios; RNA quality and integrity were assessed and ensured using Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) and RNA 6000 NanoAssay.
|
| Label |
biotin
|
| Label protocol |
Each RNA sample was amplified using the Ambion Illumina RNA amplification kit with biotin UTP labeling. T7 oligo(dT) primer was used to generate single stranded cDNA, followed by second strand synthesis to generate double-stranded cRNA, which is then column purified. In vitro transcription was performed to synthesize biotin-labeled cRNA using T7 RNA polymerase. The cRNA was then column purified and measured.
|
| |
|
| Hybridization protocol |
A total of 1500 ng of cRNA was hybridized for each array using standard Illumina protocols with streptavidin-Cy3 for detection.
|
| Scan protocol |
Slides were scanned on an Illumina Beadstation and analyzed using BeadStudio (Illumina, Inc).
|
| Description |
9237545002_A
|
| Data processing |
Samples were log-transformed and quantile normalized using Partek GS 6.6
|
| |
|
| Submission date |
Aug 04, 2015 |
| Last update date |
Jun 13, 2016 |
| Contact name |
Marten CG Winge |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Lab |
Marinkovich
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94035 |
| Country |
USA |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE71683 |
Transcriptome of K14 V12 Rac1 mice |
|
