|
| Status |
Public on Jul 29, 2015 |
| Title |
hippocampal_precursor_cell_culture_C57BL/6J_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampal precursor cell culture
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
passage number: P7
|
| Growth protocol |
Cells were cultured on poly-D-lysine/laminin-coated plates in Neurobasal media supplemented with 2 % B27, GlutaMAX, 20 ng/ml bFGF and 20 ng/ml EGF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Quiagen RNEasy Mini kit, including the on-column DNase step, following the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep-96 RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5871833108_F
|
| Data processing |
The data were normalised using quantile normalisation with beadarray (version 1.16) in R/BioConductor. This algorithm includes a log transformation. Probes were reassigned to Entrez GeneIDs by aligning the probe sequence to the mm9 genome using the BLAT algorithm. Replicate expression data for the same GeneID were then merged by calculating the mean. Illumina probe IDs and sequences used for each merged probeset are given as comma-delimited lists in the columns ‘Probes’ and ‘Probe_Sequences’ in the normalised data matrix.
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Jul 29, 2015 |
| Contact name |
Gerd Kempermann |
| E-mail(s) |
[email protected]
|
| Organization name |
German Center for Neurodegenerative Diseases (DZNE)
|
| Street address |
Tatzberg 41
|
| City |
Dresden |
| State/province |
Saxony |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE71466 |
Systems genetics analysis of a recombinant inbred mouse cell culture panel reveals Wnt pathway member Lrp6 as a regulator of adult hippocampal precursor cell proliferation |
|
