|
| Status |
Public on Oct 01, 2015 |
| Title |
wild type exposed to Congo Red 3 h_1 |
| Sample type |
RNA |
| |
|
| Source name |
yeast
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genetic background: BY4741
genotype/variation: wild type
|
| Treatment protocol |
Cells were grown overnight in liquid media YPD. The culture was refreshed to 0.2 (A600) in YPD, grown for an additional 2 h 30 min and then divided into two parts. One part continued growing under the same conditions (non-treated culture), while the other one was supplemented with Congo Red (CR) (30 µg/ml; Merck, Darmstadt, Germany), during 3 hours.
|
| Growth protocol |
Cells were grown overnight in liquid media YPD. The culture was refreshed to 0.2 (A600) in YPD, grown for an additional 2 h 30 min and then divided into two parts. One part continued growing under the same conditions (non-treated culture), while the other one was supplemented with Congo Red (CR) (30 µg/ml; Merck, Darmstadt, Germany), during 3 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from exponentially growing cells by the “mechanical disruption protocol” using the RNeasy kit (Qiagen, Hilden, Germany), following the instructions of the manufacturer. RNA concentrations were determined by measuring absorbance at 260 nm. RNA purity and integrity were assessed using RNA Nano Labchips in an Agilent 2100B Bioanalyzer (Agilent Technologies, Palo Alto, CA) following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Following Affymetrix labelling instructions
|
| |
|
| Hybridization protocol |
Following Affymetrix hybridation instructions
|
| Scan protocol |
Following Affymetrix scanning instructions
|
| Description |
All the procedure for microarrays experiments was carried out following the instructions of affymetrix for each of the steps
|
| Data processing |
CEL files from scaner were analyzed using GCRMA algorithm from Bioconductor software
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Javier Arroyo |
| E-mail(s) |
[email protected]
|
| Phone |
913941746
|
| Organization name |
Universidad Complutense de Madrid
|
| Department |
Facultad de Farmacia, Departamento de Microbiologia II
|
| Street address |
Plaza Ramón y Cajal, S/N
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE71433 |
Expresion data from yeast (wild type and gcn5 mutants) exposed to Congo Red (CR) |
|
