|
| Status |
Public on Jul 15, 2016 |
| Title |
090727_32LMD0_5077_MCL |
| Sample type |
RNA |
| |
|
| Source name |
Mantle cell lymphoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: unpurified lymph node biopsy
cell type: mixture of B, T and other cells
|
| Growth protocol |
Mononuclear cells from PB and LN single-cell suspension were isolated by density gradient centrifugation using the lymphocyte separation medium (ICN Biomedicals). Fresh cells were subject to CD19 selection by magnetic beads labeling using the MACS columns (Miltenyi Biotec). CD19 samples with purity of >96% were stored at -80°C for subsequent use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression from tumor cells in lymph node mix
|
| Data processing |
Affymetrix Expression Console was used to extract log2 RMA signal intensities of the probesets from cel files.
|
| |
|
| Submission date |
Jul 14, 2015 |
| Last update date |
Jul 15, 2016 |
| Contact name |
Delong Liu |
| E-mail(s) |
[email protected]
|
| Phone |
301-451-7129
|
| Organization name |
NHLBI/NIH
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE70910 |
Direct in vivo evidence for B-cell receptor and NF-KB activation in mantle cell lymphoma: role of the lymph node microenvironment and activating mutations. [Affymetrix] |
| GSE70927 |
Direct in vivo evidence for B-cell receptor and NF-KB activation in mantle cell lymphoma: role of the lymph node microenvironment and activating mutations. |
|
