|
| Status |
Public on Jun 30, 2007 |
| Title |
Small RNAs from Selaginella moellendorffii |
| Sample type |
MPSS |
| |
|
| Source name |
Above-ground tissues (stems and leaves)
|
| Organism |
Selaginella moellendorffii |
| Characteristics |
Wild-type, greenhouse grown S. moellendorffii
|
| Biomaterial provider |
purchased from Plant Delights, Raleigh, NC, USA
|
| Treatment protocol |
none
|
| Growth protocol |
22 degree greenhouse, 16hrs light, 8hrs dark
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the protocol of Chang et al. (1993) Plant Mol. Biol. Rep. 11: 113-116. Small RNAs were then purified by PAGE, reverse-transcribed, and sequencing using pyrosequencing (454 Life Sciences) as previously described by Axtell et al. (2006) Cell 127:565-577.
|
| |
|
| Description |
Endogenous small RNAs as determined by pyrosequencing.
|
| Data processing |
no processing - value is simple the number of times that sequence was found, out of a total of 149,586 sequence reads in the sample
|
| |
|
| Submission date |
Mar 20, 2007 |
| Last update date |
Mar 20, 2007 |
| Contact name |
Michael J Axtell |
| E-mail(s) |
[email protected]
|
| Organization name |
Pennsylvania State University
|
| Department |
Biology
|
| Lab |
Axtell
|
| Street address |
280 Mueller Lab
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL5014 |
| Series (1) |
| GSE7320 |
Small RNAs from the lycopod Selaginella moellendorffii |
|
