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Sample GSM172989

Query DataSets for GSM172989

Status

Public on Mar 04, 2008

Title

Obese Control-1

Sample type

RNA

Source name

Kidney tissue from obese rats on control diet

Organism

Rattus norvegicus

Characteristics

Zucker rats

Extracted molecule

total RNA

Extraction protocol

Trizol Reagent (Invitrogen)

Label

biotin

Label protocol

Enzo RNA Transcript Labeling

Hybridization protocol

Protocol EukGE-WS2v4_450
Wash A1 Recovery Mixes 0
Wash A1 Temperature (C) 25
Number of Wash A1 Cycles 10
Mixes per Wash A1 Cycle 2
Wash B Recovery Mixes 0
Wash B Temperature (C) 50
Number of Wash B Cycles 4
Mixes per Wash B Cycle 15
Stain Temperature (C) 25
First Stain Time (seconds) 600
Wash A2 Recovery Mixes 0
Wash A2 Temperature (C) 25
Number of Wash A2 Cycles 10
Mixes per Wash A2 Cycle 4
Second Stain Time (seconds) 600
Third Stain Time (seconds) 600
Wash A3 Recovery Mixes 0
Wash A3 Temperature (C) 30
Number of Wash A3 Cycles 15
Mixes per Wash A3 Cycle 4
Holding Temperature (C) 25
Station 1
Module 3

Scan protocol

Pixel Size 1.56
Filter 570
Scan Temperature
Scanner ID 50103010
Number of Scans 1
Scanner Type M10

Description

model for diabetes

Data processing

MAS 5.0

Submission date

Mar 05, 2007

Last update date

Mar 07, 2007

Contact name

swasti Tiwari

E-mail(s)

[email protected]

Phone

202-687-8228

Organization name

Georgetown University

Street address

4000 reservoir road, NW

City

washington DC

State/province

ZIP/Postal code

20057

Country

USA

Platform ID

GPL1355

Series (1)

GSE7193

Rosiglitazone effects on kidney gene expression

Data table header descriptions
ID_REF
VALUE	expression levels
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	402.216	P	0.000753643
AFFX-BioB-M_at	540.77	P	0.000445901
AFFX-BioB-3_at	281.082	P	0.000146581
AFFX-BioC-5_at	1401.11	P	7.00668e-05
AFFX-BioC-3_at	1308.4	P	4.42873e-05
AFFX-BioDn-5_at	2738.89	P	6.02111e-05
AFFX-BioDn-3_at	6257.89	P	9.4506e-05
AFFX-CreX-5_at	12918.1	P	5.16732e-05
AFFX-CreX-3_at	13509.4	P	4.42873e-05
AFFX-DapX-5_at	9.44782	A	0.396911
AFFX-DapX-M_at	31.4999	A	0.300606
AFFX-DapX-3_at	5.44705	A	0.916408
AFFX-LysX-5_at	8.61314	A	0.411373
AFFX-LysX-M_at	39.8912	A	0.5
AFFX-LysX-3_at	21.1639	P	0.0336773
AFFX-PheX-5_at	2.80526	A	0.824672
AFFX-PheX-M_at	1.05356	A	0.921998
AFFX-PheX-3_at	10.8646	A	0.794268
AFFX-ThrX-5_at	9.19276	A	0.686277
AFFX-ThrX-M_at	2.26077	A	0.737173

Total number of rows: 31099

Table truncated, full table size 943 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM172989.CEL.gz	4.5 Mb	(ftp)(http)	CEL
GSM172989.CHP.gz	8.0 Mb	(ftp)(http)	CHP
Processed data provided as supplementary file