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Sample GSM172988

Query DataSets for GSM172988

Status

Public on Mar 04, 2008

Title

Obese rosiglitazone-3

Sample type

RNA

Source name

Kidney tissue from obese rats on diet with rosiglitazone

Organism

Rattus norvegicus

Characteristics

Zucker rats

Extracted molecule

total RNA

Extraction protocol

Trizol Reagent (Invitrogen)

Label

biotin

Label protocol

Enzo RNA Transcript Labeling

Hybridization protocol

Protocol EukGE-WS2v4_450
Wash A1 Recovery Mixes 0
Wash A1 Temperature (C) 25
Number of Wash A1 Cycles 10
Mixes per Wash A1 Cycle 2
Wash B Recovery Mixes 0
Wash B Temperature (C) 50
Number of Wash B Cycles 4
Mixes per Wash B Cycle 15
Stain Temperature (C) 25
First Stain Time (seconds) 600
Wash A2 Recovery Mixes 0
Wash A2 Temperature (C) 25
Number of Wash A2 Cycles 10
Mixes per Wash A2 Cycle 4
Second Stain Time (seconds) 600
Third Stain Time (seconds) 600
Wash A3 Recovery Mixes 0
Wash A3 Temperature (C) 30
Number of Wash A3 Cycles 15
Mixes per Wash A3 Cycle 4
Holding Temperature (C) 25
Station 1
Module 2

Scan protocol

Pixel Size 1.56
Filter 570
Scan Temperature
Scanner ID 50103010
Number of Scans 1
Scanner Type M10

Description

model for diabetes

Data processing

MAS 5.0

Submission date

Mar 05, 2007

Last update date

Mar 07, 2007

Contact name

swasti Tiwari

E-mail(s)

[email protected]

Phone

202-687-8228

Organization name

Georgetown University

Street address

4000 reservoir road, NW

City

washington DC

State/province

ZIP/Postal code

20057

Country

USA

Platform ID

GPL1355

Series (1)

GSE7193

Rosiglitazone effects on kidney gene expression

Data table header descriptions
ID_REF
VALUE	expression levels
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	365.576	P	0.00159257
AFFX-BioB-M_at	381.977	P	0.000856509
AFFX-BioB-3_at	203.793	P	0.000972149
AFFX-BioC-5_at	1042.88	P	9.4506e-05
AFFX-BioC-3_at	912.731	P	4.42873e-05
AFFX-BioDn-5_at	1882.33	P	7.00668e-05
AFFX-BioDn-3_at	4855.24	P	9.4506e-05
AFFX-CreX-5_at	9639.34	P	5.16732e-05
AFFX-CreX-3_at	11125	P	4.42873e-05
AFFX-DapX-5_at	6.96375	A	0.368438
AFFX-DapX-M_at	9.21389	A	0.470238
AFFX-DapX-3_at	6.73197	A	0.891041
AFFX-LysX-5_at	14.4754	A	0.287743
AFFX-LysX-M_at	22.7654	A	0.485109
AFFX-LysX-3_at	21.4425	A	0.139482
AFFX-PheX-5_at	5.08881	A	0.814869
AFFX-PheX-M_at	3.82665	A	0.883887
AFFX-PheX-3_at	20.4533	A	0.672921
AFFX-ThrX-5_at	13.4161	A	0.699394
AFFX-ThrX-M_at	4.92846	A	0.724854

Total number of rows: 31099

Table truncated, full table size 941 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM172988.CEL.gz	4.6 Mb	(ftp)(http)	CEL
GSM172988.CHP.gz	8.2 Mb	(ftp)(http)	CHP
Processed data provided as supplementary file