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Sample GSM172984

Query DataSets for GSM172984

Status

Public on Mar 04, 2008

Title

lean rosiglitazone-3

Sample type

RNA

Source name

kidney tissues from lean rats on diet with rosiglitazone

Organism

Rattus norvegicus

Characteristics

Zucker rats

Extracted molecule

total RNA

Extraction protocol

Trizol Reagent (Invitrogen)

Label

biotin

Label protocol

Enzo RNA Transcript Labeling

Hybridization protocol

Protocol EukGE-WS2v4_450
Wash A1 Recovery Mixes 0
Wash A1 Temperature (C) 25
Number of Wash A1 Cycles 10
Mixes per Wash A1 Cycle 2
Wash B Recovery Mixes 0
Wash B Temperature (C) 50
Number of Wash B Cycles 4
Mixes per Wash B Cycle 15
Stain Temperature (C) 25
First Stain Time (seconds) 600
Wash A2 Recovery Mixes 0
Wash A2 Temperature (C) 25
Number of Wash A2 Cycles 10
Mixes per Wash A2 Cycle 4
Second Stain Time (seconds) 600
Third Stain Time (seconds) 600
Wash A3 Recovery Mixes 0
Wash A3 Temperature (C) 30
Number of Wash A3 Cycles 15
Mixes per Wash A3 Cycle 4
Holding Temperature (C) 25
Station 1
Module 4

Scan protocol

Pixel Size 1.56
Filter 570
Scan Temperature
Scanner ID 50103010
Number of Scans 1
Scanner Type M10

Description

model for diabetes

Data processing

MAS 5.0

Submission date

Mar 05, 2007

Last update date

Mar 07, 2007

Contact name

swasti Tiwari

E-mail(s)

[email protected]

Phone

202-687-8228

Organization name

Georgetown University

Street address

4000 reservoir road, NW

City

washington DC

State/province

ZIP/Postal code

20057

Country

USA

Platform ID

GPL1355

Series (1)

GSE7193

Rosiglitazone effects on kidney gene expression

Data table header descriptions
ID_REF
VALUE	expression levels
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	316.068	P	0.0396608
AFFX-BioB-M_at	368.504	P	0.00556451
AFFX-BioB-3_at	215.96	A	0.0676785
AFFX-BioC-5_at	1004.79	P	0.000509415
AFFX-BioC-3_at	807.763	P	8.14279e-05
AFFX-BioDn-5_at	1481.93	P	0.000296708
AFFX-BioDn-3_at	5225.59	P	0.000146581
AFFX-CreX-5_at	8452.44	P	4.42873e-05
AFFX-CreX-3_at	10199.5	P	4.42873e-05
AFFX-DapX-5_at	41.887	A	0.0956669
AFFX-DapX-M_at	115.7	A	0.354453
AFFX-DapX-3_at	16.5457	A	0.783476
AFFX-LysX-5_at	5.26618	A	0.852061
AFFX-LysX-M_at	64.7868	A	0.470241
AFFX-LysX-3_at	33.5672	A	0.0956669
AFFX-PheX-5_at	7.22279	A	0.876428
AFFX-PheX-M_at	8.97858	A	0.897835
AFFX-PheX-3_at	16.884	A	0.9273
AFFX-ThrX-5_at	12.7418	A	0.932322
AFFX-ThrX-M_at	12.3466	A	0.843268

Total number of rows: 31099

Table truncated, full table size 936 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM172984.CEL.gz	4.6 Mb	(ftp)(http)	CEL
GSM172984.CHP.gz	8.1 Mb	(ftp)(http)	CHP
Processed data provided as supplementary file