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Sample GSM172983

Query DataSets for GSM172983

Status

Public on Mar 04, 2008

Title

lean rosiglitazone 2

Sample type

RNA

Source name

kidney tissues from lean rats on diet with rosiglitazone

Organism

Rattus norvegicus

Characteristics

Zucker rats

Extracted molecule

total RNA

Extraction protocol

Trizol Reagent (Invitrogen)

Label

biotin

Label protocol

Enzo RNA Transcript Labeling

Hybridization protocol

Protocol EukGE-WS2v4_450
Wash A1 Recovery Mixes 0
Wash A1 Temperature (C) 25
Number of Wash A1 Cycles 10
Mixes per Wash A1 Cycle 2
Wash B Recovery Mixes 0
Wash B Temperature (C) 50
Number of Wash B Cycles 4
Mixes per Wash B Cycle 15
Stain Temperature (C) 25
First Stain Time (seconds) 600
Wash A2 Recovery Mixes 0
Wash A2 Temperature (C) 25
Number of Wash A2 Cycles 10
Mixes per Wash A2 Cycle 4
Second Stain Time (seconds) 600
Third Stain Time (seconds) 600
Wash A3 Recovery Mixes 0
Wash A3 Temperature (C) 30
Number of Wash A3 Cycles 15
Mixes per Wash A3 Cycle 4
Holding Temperature (C) 25
Station 1
Module 3

Scan protocol

Pixel Size 1.56
Filter 570
Scan Temperature
Scanner ID 50103010
Number of Scans 1
Scanner Type M10

Description

model for diabetes

Data processing

MAS 5.0

Submission date

Mar 05, 2007

Last update date

Mar 07, 2007

Contact name

swasti Tiwari

E-mail(s)

[email protected]

Phone

202-687-8228

Organization name

Georgetown University

Street address

4000 reservoir road, NW

City

washington DC

State/province

ZIP/Postal code

20057

Country

USA

Platform ID

GPL1355

Series (1)

GSE7193

Rosiglitazone effects on kidney gene expression

Data table header descriptions
ID_REF
VALUE	expression levels
ABS_CALL	present absent calls
DETECTION P-VALUE	P-values for present absent call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	408.227	P	0.000753643
AFFX-BioB-M_at	525.972	P	0.00110197
AFFX-BioB-3_at	273.368	P	0.000581214
AFFX-BioC-5_at	1328.74	P	0.00010954
AFFX-BioC-3_at	1317.68	P	4.42873e-05
AFFX-BioDn-5_at	2220.28	P	6.02111e-05
AFFX-BioDn-3_at	6465.56	P	8.14279e-05
AFFX-CreX-5_at	11597.5	P	5.16732e-05
AFFX-CreX-3_at	14691.8	P	4.42873e-05
AFFX-DapX-5_at	17.5781	A	0.239063
AFFX-DapX-M_at	5.05386	A	0.686277
AFFX-DapX-3_at	4.74505	A	0.921998
AFFX-LysX-5_at	14.9091	A	0.440646
AFFX-LysX-M_at	17.1753	A	0.645559
AFFX-LysX-3_at	30.1569	M	0.0584438
AFFX-PheX-5_at	4.24078	A	0.804734
AFFX-PheX-M_at	4.31362	A	0.932322
AFFX-PheX-3_at	15.5727	A	0.760937
AFFX-ThrX-5_at	39.6526	A	0.354453
AFFX-ThrX-M_at	19.3102	A	0.48511

Total number of rows: 31099

Table truncated, full table size 940 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM172983.CEL.gz	4.4 Mb	(ftp)(http)	CEL
GSM172983.CHP.gz	7.8 Mb	(ftp)(http)	CHP
Processed data provided as supplementary file