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Sample GSM1726319 Query DataSets for GSM1726319
Status Public on Jun 30, 2015
Title responder TAG# F8E1
Sample type SRA
 
Source name ovary
Organism Anguilla anguilla
Characteristics tissue: ovary
phenotype: responder
Extracted molecule total RNA
Extraction protocol Tissues were taken from anterior, posterior and middle parts of gonad from each individual, and stored in RNAlater (Ambion) at -80ºC till RNA extraction. The different parts of gonad tissue of each individual were pooled. Total RNA was isolated from the pooled samples using the Qiagen miRNeasy kit according to the manufacturer’s instructions (Qiagen), and analyzed with an Agilent Bioanalyzer 2100 total RNA Nano series II chip (Agilent). A transcriptome library for each individual was prepared from 1 microgram total RNA, using the Illumina mRNAseq Sample Preparation Kit following the manufacturer’s instructions (Illumina Inc.).
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Origin of specimen: commercial eel farm (Passie voor Vis, Sevenum, The Netherlands)
Data processing Supplementary_files_format_and_content: Microsoft Office Excel file (.xlsx) with the following columns: id; Name; Best hit; Description; Alternate descriptions; Min e-value; Similarity; GO molecular function; GO biological process; GO cellular component; Transcripts; Longest transcript length; Total length; sample 1; sample 2; sample 3; sample 4; sample 5; sample 6; BaseMean; baseMeanA; baseMeanB; foldChange; log2FoldChange; pval; padj
 
Submission date Jun 29, 2015
Last update date May 15, 2019
Contact name Ron P Dirks
E-mail(s) [email protected]
Phone +31-71-2074610
Organization name Future Genomics Technologies BV
Street address Sylviusweg 74
City Leiden
ZIP/Postal code 2333 BE
Country Netherlands
 
Platform ID GPL20640
Series (1)
GSE70382 Changes in ovarian gene expression profiles in maturing European eel (Anguilla anguilla) (responders and non-responders)
Relations
BioSample SAMN03801235
SRA SRX1075743

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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