NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1717378 Query DataSets for GSM1717378
Status Public on Jun 22, 2015
Title Mice_Lung_IAV_36h
Sample type RNA
 
Source name lung, 36hr
Organism Mus musculus
Characteristics strain: C57BL/6
gender: male
age: 6w
infection: IAV
time: 36h
tissue: lung
Treatment protocol Mice were treated with 50µl of influenza A (50 µl of 10-6 TCID50/µl) via retropharyngeal instillation for 24h or 36h.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified using mirVana™ miRNA Isolation Kit (Cat#AM1560, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100(Agilent technologies, Santa Clara, CA, US). Three mice were performed at each time (24 or 36 hours) and RNA from different donors was mixed before determination.
Label Cy3
Label protocol miRNA molecular in total RNA was labeled by miRNA Complete Labeling and Hyb Kit(Cat#5190-0456, Agilent technologies, Santa Clara, CA, US)followed the manufacturer’s instructions, labeling section.
 
Hybridization protocol Each slide was hybridized with 100ng Cy3-labeled RNA using miRNA Complete Labeling and Hyb Kit(Cat#5190-0456, Agilent technologies, Santa Clara, CA, US)in hybridization Oven(Cat#G2545A, Agilent technologies, Santa Clara, CA, US)at 55℃,20rpm for 20 hours according to the manufacturer’s instructions, hybridization section. After hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat#5188-5327, Agilent technologies, Santa Clara, CA, US).
Scan protocol Slides were scanned by Agilent Microarray Scanner(Cat#G2565BA,Agilent technologies, Santa Clara, CA, US).
Description miRNA expression in IAV infection mice at 36h
GM0003
Data processing Data was read by Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US)with default settings(Scan resolution=5μm, PMT 100%,5% )
Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0(Agilent technologies, Santa Clara, CA, US).
 
Submission date Jun 22, 2015
Last update date Jun 22, 2015
Contact name Meng Guo
E-mail(s) [email protected]
Phone +86-13916404693
Organization name ShangHai Changzheng Hospital
Department Organ transplantation center
Street address No. 415, Fengyang Road, Huangpu District
City Shanghai
ZIP/Postal code 200433
Country China
 
Platform ID GPL17912
Series (1)
GSE70125 miRNA profile in mice lung after influenza A infection

Data table header descriptions
ID_REF
VALUE Quantile-normalized signal
FLAGS

Data table
ID_REF VALUE FLAGS
Blank -3.3013506 A
NC1_00000197 -3.3013506 A
NC1_00000215 -3.3013506 A
NC2_00079215 -3.3013506 A
NC2_00092197 -3.3013506 A
NC2_00106057 -3.3013506 A
NC2_00122731 -3.3013506 A
NegativeControl -3.3013506 A
dmr_285 9.892676 P
dmr_3 13.708923 P
dmr_308 -3.3013506 A
dmr_316 -3.3013506 A
dmr_31a 8.785011 P
dmr_6 12.864699 P
hur_1 14.965093 P
hur_2 16.347246 P
hur_4 11.76222 P
hur_5 -3.3013506 A
hur_6 14.509013 P
miRNABrightCorner30 -3.3013506 A

Total number of rows: 1268

Table truncated, full table size 34 Kbytes.




Supplementary file Size Download File type/resource
GSM1717378_GM0003.txt.gz 7.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap