|
| Status |
Public on Jul 01, 2015 |
| Title |
adhE1_alcohologenesis_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Chemostat culture
|
| Organism |
Clostridium acetobutylicum ATCC 824 |
| Characteristics |
genotype/variation: ΔadhE1
condition: alcohologenesis
|
| Treatment protocol |
Immediately frozen in liquid nitrogen
|
| Growth protocol |
The culture conditions described previously by Vasconcelos et al. (Vasconcelos I, et al. J Bacteriol. 1994; 176, 1443-1450.) and Girbal et al. (Girbal L, Soucaille P. J Bacteriol. 1995; 176,6433-6438.) were used for the phosphate-limited continuous culture of C. acetobutylicum, fed with a constant total carbon amount of 995 mM. The cultures were maintained under acidogenesis (pH 6.3, 995 mM of carbon from glucose), solventogenesis (pH 4.4, 995 mM of carbon from glucose) and alcohologenesis (pH 6.3, 498 mM of carbon from glucose and 498 mM of carbon from glycerol).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using an RNeasy Midi kit (Qiagen, Courtaboeuf, France) following the manufacturer's instructions with supplementation of DNase treatment using RNase-Free DNase Set (Qiagen). RNA quantity and quality evaluations were performed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Massy, France) and a NanoDrop ND-1000 spectrophotometer (Labtech France, Paris, France) at 260 nm and 280 nm.
|
| Label |
Cy3
|
| Label protocol |
The RNAs were labeled with a Low Input Quick Amp Labeling kit (Agilent)
|
| |
|
| Hybridization protocol |
Hybridization followed a one-color microarray-based gene expression analysis protocol (Agilent)
|
| Scan protocol |
The slides were scanned using a Tecan MS200 scanner.
|
| Data processing |
The scanned slides were analyzed using Feature Extraction V.11.5.1.1.
|
| |
|
| Submission date |
Jun 18, 2015 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Philippe Soucaille |
| Organization name |
INSA Toulouse
|
| Street address |
135 avenue de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31077 |
| Country |
France |
| |
|
| Platform ID |
GPL10908 |
| Series (1) |
| GSE69973 |
Quantitative transcriptomic analysis of wild type and metabolic mutants of Clostrdidium acetobutylicum ATCC824 under different physiological conditions |
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