|
| Status |
Public on Sep 27, 2015 |
| Title |
Nos3-/- mice, right ventricle, 1 |
| Sample type |
RNA |
| |
|
| Source name |
E14.5 right ventricle heart tissues from Nos3-/- mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: right ventricle heart tissue
genotype/variation: Nos3-/-
age: E14.5
strain: B6.129P2-Nos3tm1unc/J
|
| Treatment protocol |
Left ventricle and right ventricle tissues were collect at E14.5, washed with sterile PBS, and pooled into triplicate samples on ice. Samples were then flash-frozen in liquid nitrogen and stored at -80 degrees Celsius until RNA harvest.
|
| Growth protocol |
The study samples consisted of heart tissues (left ventricle and right ventricle) collected from wild type CD1 and Nos3-/- mice at embryonic day 14.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
right ventricle, Nos3-/-
|
| Data processing |
The data were analyzed with R/Bioconductor using RMA.
|
| |
|
| Submission date |
May 28, 2015 |
| Last update date |
Sep 27, 2015 |
| Contact name |
XING LI |
| E-mail(s) |
[email protected]
|
| Organization name |
Mayo Clinic
|
| Department |
Health Sciences Research
|
| Street address |
200 1st street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE69315 |
Nos3-/- iPSCs model concordant signatures of in utero cardiac pathogenesis [tissue] |
| GSE69317 |
Nos3-/- iPSCs model concordant signatures of in utero cardiac pathogenesis |
|
