|
| Status |
Public on Feb 27, 2017 |
| Title |
Wild type 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Kidney RNA
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed background
age: embryonic day 18.5
genotype: RBPJ f/f;Rosa +/Eyfp
tissue: kidney
|
| Treatment protocol |
HoxB7Cre was used to inactivate RBPJ floxed alleles and hence Notch signlaing in the collecting ducts
|
| Growth protocol |
mice were housed in cages with a 12 hour night and 12 hour day cycle and fed regular rodent chow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy Mini kits from Qiagen, treated with DNAse. Quality of RNA was verified using BioAnalyzer (Agilent Tech.)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using 500ng of RNA and with Illumina® RNA Amplification Kit from Ambion (Austin, TX, USA)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol: The labeled cRNA (1500ng for Mouse) was hybridized overnight at 58°C onto the Expression BeadChip mouse GW-6 (>46,000 gene transcripts; Illumina, San Diego, CA, USA) according to the manufacturer's instructions
|
| Scan protocol |
Standard Illumina scanning protocol:. BeadChips were subsequently washed and developed with fluorolink streptavidin- Cy3 (GE Healthcare). BeadChips were scanned with an Illumina BeadArray Reader. Data was generated from Imagedata using Illumina software, GenomeStudio.
|
| Description |
whole kidney from wild type mouse 2
|
| Data processing |
The data were normalised using quantile normalisation
|
| |
|
| Submission date |
Apr 29, 2015 |
| Last update date |
Feb 27, 2017 |
| Contact name |
Kameswaran Surendran |
| E-mail(s) |
[email protected]
|
| Phone |
6053126415
|
| Organization name |
Sandord Research
|
| Department |
Children's Research Center
|
| Street address |
2310 East 60th Street North
|
| City |
Sioux Falls |
| State/province |
SD |
| ZIP/Postal code |
57104 |
| Country |
USA |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE68389 |
Identification of down-stream targets of Notch signaling within the developing mosue kidney collecting ducts. |
|
