NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM16655 Query DataSets for GSM16655
Status Public on Feb 11, 2004
Title RatLung_H6h_5
Sample type RNA
 
Channel 1
Source name Lung of hemorrhaged animal 6h
Organism Rattus norvegicus
Extracted molecule total RNA
 
Channel 2
Source name Pooled RNA of equal amounts from 10 organs (liver, lung, kidney, spleen, heart, skeletal muscle, skin, jejunum, and brain) of untreated control animals
Organism Rattus norvegicus
Extracted molecule total RNA
 
 
Description Male Sprague-Dawley rats weighing 280-300 g were anesthetized with 1.75% to 2.5% isofluorane and instrumented with two arterial catheters, one externalized for blood withdrawal and the other (not externalized) attached to the Data Sciences International (DSI) PhysioTel (St. Paul, MN, model C50-PXT) monitoring device placed intraperitoneally and used in monitoring blood pressure, temperature, and electrocardiogram data via telemetry. After 7 to 10 days of recovery, and only after the animals had begun to gain weight, the experiment was performed. At the beginning of hemorrhage (H), blood was withdrawn manually through the externalized catheter with 1-ml syringes at l ml/min. Total blood volume was estimated to be 6.5% of the body weight and 40% of this (2.6 ml/100g) was removed to effect the hemorrhage.
Keywords = rat lung hemorrhage
 
Submission date Feb 06, 2004
Last update date May 28, 2005
Contact name Phillip Bowman
E-mail(s) [email protected]
Phone 210-916-4297
Organization name Institute of Surgical Research
Street address 3400 Rawley E. Chambers Ave
City San Antonio
State/province TX
ZIP/Postal code 78234-6315
Country USA
 
Platform ID GPL968
Series (1)
GSE1034 Temporal Response of Rat Lung to Hemorrhage

Data table header descriptions
ID_REF
VALUE log ratio (log2 of PRE_VALUE)
N635_MEDIAN The normalized median intensity of Cy5 calculated by subtracting the Cy5 median background intensity taken from a standard area around the spot from the Cy5 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array.
N532_MEDIAN The normalized median intensity of Cy3 calculated by subtracting the Cy3 median background intensity taken from a standard area around the spot from the Cy3 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array.
F635_MEDIAN Cy5 channel median intensity of the spot.
F635_MEAN Cy5 channel average intensity of the spot.
F635_SD The standard deviation calculated for the Cy5 channel mean intensity of the spot.
B635_MEDIAN Cy5 channel background median intensity around the spot.
B635_MEAN Cy5 channel background average intensity around the spot.
B635_SD The standard deviation calculated for the Cy5 channel background average intensity around the spot.
F635_QUALITY Cy5 channel percentage of pixels in the spot more than one standard deviation above background.
F532_MEDIAN Cy3 channel median intensity of the spot.
F532_MEAN Cy3 channel average intensity of the spot.
F532_SD The standard deviation calculated for the Cy3 channel mean intensity of the spot.
B532_MEDIAN Cy3 channel background median intensity around the spot.
B532_MEAN Cy3 channel background average intensity around the spot.
B532_SD The standard deviation calculated for the Cy3 channel background average intensity around the spot.
F543_QUALITY Cy3 channel percentage of pixels in the spot more than one standard deviation above background.
FLAGS The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity.
PRE_VALUE The ratio of normalized median intensity of Cy5 (N635_MEDIAN) to normalized median intensity of Cy3 (N532_MEDIAN). These values were further processed before statistical analysis. Please refer to the paper and its supplement for the detailed data analysis.

Data table
ID_REF VALUE N635_MEDIAN N532_MEDIAN F635_MEDIAN F635_MEAN F635_SD B635_MEDIAN B635_MEAN B635_SD F635_QUALITY F532_MEDIAN F532_MEAN F532_SD B532_MEDIAN B532_MEAN B532_SD F543_QUALITY FLAGS PRE_VALUE
1 0.1110313 1.162666979 1.079398266 2915 3002 1260 556 1198 3441 18 2845 2777 809 662 1871 6899 0 0 1.08
2 -0.0439433 1.138023762 1.175817259 2827 2944 1119 518 584 415 97 3009 2928 722 630 679 335 97 0 0.97
3 0.6780723 0.298182926 0.185915597 1127 1233 617 521 585 412 66 1020 1052 286 644 694 429 42 0 1.60
4 1.2448873 0.412527453 0.174048644 1427 1474 686 589 1018 3482 0 1075 1123 325 723 1417 5388 0 0 2.37
5 -1.0892673 1.780225997 3.768252032 4114 4210 1396 501 685 1274 97 8218 8996 4068 596 857 2360 98 0 0.47
6 -0.9159363 1.994129121 3.77121877 4519 4645 1542 472 534 377 99 8174 9028 3994 546 573 224 100 0 0.53
7 -0.0145003 0.385419914 0.389631623 1239 1304 812 457 528 378 72 1330 1352 517 542 573 274 93 0 0.99
8 -0.3584543 0.332190565 0.426715851 1123 1213 664 448 524 386 67 1388 1401 415 525 546 198 94 0 0.78
9 -1.0291463 0.198131465 0.405454227 862 897 490 459 523 390 51 1348 1601 1076 528 588 1175 21 0 0.49
10 -1.0588943 0.213410259 0.445505194 890 953 532 456 526 376 56 1428 1647 1028 527 544 212 98 0 0.48
11 -0.9434163 0.151802217 0.292718174 763 821 477 455 521 405 40 1113 1980 3145 520 545 477 61 0 0.52
12 -0.9159363 0.177431163 0.335241422 806 866 530 446 527 412 46 1211 3604 8898 533 561 493 73 0 0.53
13 -0.5994623 0.256782321 0.387653798 984 1041 593 462 524 380 63 1328 2434 3675 543 570 351 90 0 0.66
14 -0.4150373 0.294732875 0.3955651 1064 1170 614 466 529 372 67 1347 2367 3703 546 566 238 97 0 0.75
15 -1.1520033 0.113358798 0.251183838 695 770 477 465 524 376 35 1055 1979 3357 546 559 182 87 0 0.45
16 -0.8624963 0.142930659 0.25909514 758 822 475 467 537 393 40 1059 1135 534 535 551 191 87 0 0.55
17 -1.2175913 0.155745132 0.364908805 847 910 494 531 608 463 40 1354 1370 337 615 710 805 41 0 0.43
18 -0.6896603 0.192709957 0.312990885 906 1123 2082 514 589 438 45 1253 1269 662 619 670 506 60 0 0.62
19 -0.2009133 0.632837813 0.725861958 1876 1934 906 592 964 2502 10 2230 2503 2283 762 1533 5679 2 0 0.87
20 -0.0291463 0.543136503 0.552307771 1703 1817 903 601 1915 7041 0 1942 3252 5326 825 2847 9475 5 0 0.98

Total number of rows: 13056

Table truncated, full table size 1328 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap