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Sample GSM1664558 Query DataSets for GSM1664558
Status Public on Jun 02, 2015
Title Vehicle Ctrl - Glucagon_1
Sample type RNA
 
Channel 1
Source name Vehicle Ctrl - Glucagon
Organism Homo sapiens
Characteristics treatment: -GCG
tissue: Liver
Extracted molecule total RNA
Extraction protocol After glucagon stimulation cells will be harvested in Qiazol & RNA will be isolated, DNAse treated, concentrated by ethanol precipitation and resuspended in nuclease free water.
Label Cy5
Label protocol Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal human reference.
 
Channel 2
Source name Universal human reference (UHR)
Organism Homo sapiens
Characteristics sample type: Universal human reference (UHR)
Extracted molecule total RNA
Extraction protocol After glucagon stimulation cells will be harvested in Qiazol & RNA will be isolated, DNAse treated, concentrated by ethanol precipitation and resuspended in nuclease free water.
Label Cy3
Label protocol Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal human reference.
 
 
Hybridization protocol Agilent In Situ Hybridization Kit Plus was used where Cy-dye labeled control and test samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, the arrays were washed twice, dried and then scanned.
Scan protocol Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software version 10.7.
Data processing Agilent Feature Extraction Software version 10.7 was used for background subtraction and LOWESS normalization.
 
Submission date Apr 21, 2015
Last update date Jun 02, 2015
Contact name Matthew Brauer
E-mail(s) [email protected]
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
 
Platform ID GPL6480
Series (2)
GSE68144 Primary hepatocytes with glucagon treatment expression profiling
GSE68145 Glucagon Couples Hepatic Amino Acid Catabolism to mTOR-dependent Regulation of α-cell Mass

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_24_P66027 0.103690529
A_32_P77178 -0.255746627
A_23_P212522 -0.487210091
A_24_P934473 -0.227153432
A_24_P9671 0.432148545
A_32_P29551 -0.423934248
A_24_P801451 -0.89797077
A_32_P30710 0.073513391
A_32_P89523 0.543071423
A_24_P704878 -0.02720387
A_32_P86028 0.151782358
A_24_P470079 -0.033820628
A_23_P65830 -0.120453563
A_23_P109143 -0.41712805
A_24_P595567 -0.294250308
A_24_P391591 -0.918952598
A_24_P799245 -0.095834408
A_24_P932757 0.227274278
A_24_P835500 1.15699363
A_23_P54340 0.087054003

Total number of rows: 41000

Table truncated, full table size 1002 Kbytes.




Supplementary file Size Download File type/resource
GSM1664558_Agilent_251485063911_S01_GE2_107_Sep09_1_1.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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