|
Status |
Public on Jun 02, 2015 |
Title |
Vehicle Ctrl - Glucagon_1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Vehicle Ctrl - Glucagon
|
Organism |
Homo sapiens |
Characteristics |
treatment: -GCG tissue: Liver
|
Extracted molecule |
total RNA |
Extraction protocol |
After glucagon stimulation cells will be harvested in Qiazol & RNA will be isolated, DNAse treated, concentrated by ethanol precipitation and resuspended in nuclease free water.
|
Label |
Cy5
|
Label protocol |
Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal human reference.
|
|
|
Channel 2 |
Source name |
Universal human reference (UHR)
|
Organism |
Homo sapiens |
Characteristics |
sample type: Universal human reference (UHR)
|
Extracted molecule |
total RNA |
Extraction protocol |
After glucagon stimulation cells will be harvested in Qiazol & RNA will be isolated, DNAse treated, concentrated by ethanol precipitation and resuspended in nuclease free water.
|
Label |
Cy3
|
Label protocol |
Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal human reference.
|
|
|
|
Hybridization protocol |
Agilent In Situ Hybridization Kit Plus was used where Cy-dye labeled control and test samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, the arrays were washed twice, dried and then scanned.
|
Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software version 10.7.
|
Data processing |
Agilent Feature Extraction Software version 10.7 was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Apr 21, 2015 |
Last update date |
Jun 02, 2015 |
Contact name |
Matthew Brauer |
E-mail(s) |
[email protected]
|
Organization name |
Genentech, Inc.
|
Department |
Bioinformatics
|
Street address |
1 DNA Way
|
City |
South San Francisco |
State/province |
California |
ZIP/Postal code |
94080 |
Country |
USA |
|
|
Platform ID |
GPL6480 |
Series (2) |
GSE68144 |
Primary hepatocytes with glucagon treatment expression profiling |
GSE68145 |
Glucagon Couples Hepatic Amino Acid Catabolism to mTOR-dependent Regulation of α-cell Mass |
|