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Sample GSM16639 Query DataSets for GSM16639
Status Public on Feb 11, 2004
Title RatLung_Con_3
Sample type RNA
 
Channel 1
Source name Lung of control animal
Organism Rattus norvegicus
Extracted molecule total RNA
 
Channel 2
Source name Pooled RNA of equal amounts from 10 organs (liver, lung, kidney, spleen, heart, skeletal muscle, skin, jejunum, and brain) of untreated control animals
Organism Rattus norvegicus
Extracted molecule total RNA
 
 
Description Male Sprague-Dawley rats weighing 280-300 g were anesthetized with 1.75% to 2.5% isofluorane and instrumented with two arterial catheters, one externalized for blood withdrawal and the other (not externalized) attached to the Data Sciences International (DSI) PhysioTel (St. Paul, MN, model C50-PXT) monitoring device placed intraperitoneally and used in monitoring blood pressure, temperature, and electrocardiogram data via telemetry. After 7 to 10 days of recovery, and only after the animals had begun to gain weight, the experiment was performed. At the beginning of hemorrhage (H), blood was withdrawn manually through the externalized catheter with 1-ml syringes at l ml/min. Total blood volume was estimated to be 6.5% of the body weight and 40% of this (2.6 ml/100g) was removed to effect the hemorrhage.
Keywords = rat lung hemorrhage
 
Submission date Feb 06, 2004
Last update date May 28, 2005
Contact name Phillip Bowman
E-mail(s) [email protected]
Phone 210-916-4297
Organization name Institute of Surgical Research
Street address 3400 Rawley E. Chambers Ave
City San Antonio
State/province TX
ZIP/Postal code 78234-6315
Country USA
 
Platform ID GPL968
Series (1)
GSE1034 Temporal Response of Rat Lung to Hemorrhage

Data table header descriptions
ID_REF
VALUE log ratio (log2 of PRE_VALUE)
N635_MEDIAN The normalized median intensity of Cy5 calculated by subtracting the Cy5 median background intensity taken from a standard area around the spot from the Cy5 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array.
N532_MEDIAN The normalized median intensity of Cy3 calculated by subtracting the Cy3 median background intensity taken from a standard area around the spot from the Cy3 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array.
F635_MEDIAN Cy5 channel median intensity of the spot.
F635_MEAN Cy5 channel average intensity of the spot.
F635_SD The standard deviation calculated for the Cy5 channel mean intensity of the spot.
B635_MEDIAN Cy5 channel background median intensity around the spot.
B635_MEAN Cy5 channel background average intensity around the spot.
B635_SD The standard deviation calculated for the Cy5 channel background average intensity around the spot.
F635_QUALITY Cy5 channel percentage of pixels in the spot more than one standard deviation above background.
F532_MEDIAN Cy3 channel median intensity of the spot.
F532_MEAN Cy3 channel average intensity of the spot.
F532_SD The standard deviation calculated for the Cy3 channel mean intensity of the spot.
B532_MEDIAN Cy3 channel background median intensity around the spot.
B532_MEAN Cy3 channel background average intensity around the spot.
B532_SD The standard deviation calculated for the Cy3 channel background average intensity around the spot.
F543_QUALITY Cy3 channel percentage of pixels in the spot more than one standard deviation above background.
FLAGS The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity.
PRE_VALUE The ratio of normalized median intensity of Cy5 (N635_MEDIAN) to normalized median intensity of Cy3 (N532_MEDIAN). These values were further processed before statistical analysis. Please refer to the paper and its supplement for the detailed data analysis.

Data table
ID_REF VALUE N635_MEDIAN N532_MEDIAN F635_MEDIAN F635_MEAN F635_SD B635_MEDIAN B635_MEAN B635_SD F635_QUALITY F532_MEDIAN F532_MEAN F532_SD B532_MEDIAN B532_MEAN B532_SD F543_QUALITY FLAGS PRE_VALUE
1 0.0976113 1.661060421 1.549193445 4318 4271 1924 645 776 900 90 4242 4038 1625 798 884 1266 85 0 1.07
2 0.0703893 1.718057592 1.631061392 4393 4527 1523 594 669 515 99 4394 4476 1124 768 787 251 99 0 1.05
3 0.8237493 0.781223134 0.442176875 2302 2510 1547 574 693 870 82 1745 1840 1093 761 800 518 84 0 1.77
4 0.8155753 0.877123136 0.497955036 2538 2621 1211 599 677 481 93 1860 1900 477 753 807 668 87 0 1.76
5 0.1110313 3.392688769 3.145168574 8153 8282 2604 652 1490 6502 65 7795 7783 2061 802 1383 5176 84 0 1.08
6 0.1375043 3.520253866 3.213541804 8478 8714 2697 695 884 1306 99 7927 8161 1973 783 894 1528 99 0 1.10
7 0.2016343 1.185179277 1.033245454 3242 3282 1577 621 704 510 90 3019 2983 1192 722 744 259 92 0 1.15
8 0.1505603 1.366575036 1.231167962 3616 3809 1820 594 901 3587 36 3461 3661 1470 723 830 1941 83 0 1.11
9 -0.9714313 0.455525012 0.894249875 1601 1763 974 594 693 532 72 2701 2674 707 713 744 293 97 0 0.51
10 -0.7369663 0.597113223 0.989162714 1871 2129 1367 551 644 505 82 2870 3120 1394 670 699 280 99 0 0.60
11 -0.0892673 0.69165615 0.735911869 2107 2221 1300 577 686 634 86 2343 2405 851 707 743 382 99 0 0.94
12 0.0426443 0.778056624 0.754354648 2272 2474 1937 551 657 606 88 2393 2563 1877 716 738 301 99 0 1.03
13 -0.4540323 0.419336332 0.576674215 1488 1614 956 560 1231 5331 0 1984 2064 769 702 1376 5826 0 0 0.73
14 -0.4540323 0.533330674 0.731863454 1720 1905 1037 541 627 482 82 2292 2440 913 664 689 251 99 0 0.73
15 0.2141253 0.741415586 0.64099903 2211 2297 1085 572 654 506 87 2132 2171 599 706 731 264 99 0 1.16
16 0.6690273 1.032734461 0.64909586 2839 3107 1698 555 652 621 94 2128 2565 1749 685 721 358 99 0 1.59
17 -0.4941093 0.448287276 0.62705449 1576 1688 902 585 742 1615 21 2171 2278 1315 777 828 706 90 0 0.71
18 -0.0740013 0.648229734 0.684631947 2012 2897 6300 578 723 1701 38 2238 2862 4588 716 770 1224 71 0 0.95
19 0.7311833 1.467450982 0.886602869 3831 4021 1418 587 685 542 99 2700 2875 1241 729 779 372 99 0 1.66
20 0.4329593 1.333552865 0.986013947 3550 3711 1366 601 697 547 99 2944 3317 2640 752 787 358 98 0 1.35

Total number of rows: 13056

Table truncated, full table size 1342 Kbytes.




Supplementary data files not provided
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