|
| Status |
Public on Aug 24, 2015 |
| Title |
ΔcspC x WT_stationary phase_rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT_stationary phase
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype/variation: WT
growth phase: stationary phase
|
| Treatment protocol |
Cells were harvested from cultures at log or stationary phase with no additional treatment
|
| Growth protocol |
Overnight cultures were diluted in fresh complex medium and grown to log or stationary phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
standard Cy3-dCTP incorporation
|
| |
|
| Channel 2 |
| Source name |
ΔcspC_stationary phase
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype/variation: cspC mutant
growth phase: stationary phase
|
| Treatment protocol |
Cells were harvested from cultures at log or stationary phase with no additional treatment
|
| Growth protocol |
Overnight cultures were diluted in fresh complex medium and grown to log or stationary phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
standard Cy5-dCTP incorporation
|
| |
|
| |
| Hybridization protocol |
Agilent protocol
|
| Scan protocol |
Agilent protocol
|
| Data processing |
Data were extracted and normalized using Agilent Feature Extraction Software 9.0. Agilent output files were then opened using Excel and the log10 values extracted with no additional processing or filtering
|
| |
|
| Submission date |
Apr 06, 2015 |
| Last update date |
Aug 25, 2015 |
| Contact name |
Juliana da Silva Santos |
| E-mail(s) |
[email protected]
|
| Phone |
551130917299
|
| Organization name |
Institute of Biomedical Sciences from University of São Paulo
|
| Department |
Microbiology
|
| Lab |
222
|
| Street address |
Avenida Professor Lineu Prestes, 1374
|
| City |
São Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL10469 |
| Series (1) |
| GSE61726 |
Stationary phase stimulon and CspC regulon |
|
