|
| Status |
Public on Apr 07, 2015 |
| Title |
WT 1 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse, quadriceps
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
tissue: Quadriceps
gender: male
age: 17-18 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from the quadriceps of WT and NF90-NF45 dbTg mouse using miRNeasy Mini Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT Amplification Kit Module 1(Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentaion, single-stranded cDNA were hybridized for 20 hours at 48 ℃ with MoGene2.1ST Array Strip (Affymetrix) and GeneChip Expression 3'-Amplification Reagents Hybridization Control kit (Affymetrix).
|
| Scan protocol |
The array was scanned using Imaging Station (Affymetrix) according to the manufacture's instructions..
|
| Description |
RMA expression value derived from Expression Console software; RMA-sketch analysis
|
| Data processing |
Arrays were summarized and normalized with Expression Console using RMA-sketch.
|
| |
|
| Submission date |
Apr 06, 2015 |
| Last update date |
Apr 07, 2015 |
| Contact name |
Shuji Sakamoto |
| E-mail(s) |
[email protected]
|
| Organization name |
Kochi University
|
| Department |
Science Research Center
|
| Lab |
Laboratory of Molecular Biology
|
| Street address |
Oko-cho, Kohasu
|
| City |
Nankoku |
| State/province |
Kochi |
| ZIP/Postal code |
783-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE67591 |
Whole genome expression microarray analysis in quadriceps of wild-type and NF90-NF45 double transgenic mouse |
|
