|
| Status |
Public on Dec 01, 2018 |
| Title |
G599-12 thalamus |
| Sample type |
RNA |
| |
|
| Source name |
thalamus of suicide victim
|
| Organism |
Homo sapiens |
| Characteristics |
group: suicide victim
individual: G599-12
brain region: thalamus
rin: 7.1
hybridiziation date: 2014-01-17
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After hippocampus, amygdala, thalamus and prefrontal cortex tissue was homogenized, total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies). Genomic DNA was removed using the RNeasy Plus kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
100ng total RNA from each sample was processed with the WT PLUS reagent kit (Affymetrix, Santa Clara, CA) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed using the Affymetrix Fluidics Station 450 with program FS450_0001.
|
| Scan protocol |
Microarrays were scanned using an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
suicide victim G599-12 thalamus
|
| Data processing |
Data were preprocessed with the Expression Console v1.2.1.20 software (Affymetrix); background correction, normalization and gene-level probeset summary were performed with the RMA sketch workflow using default parameter settings.
|
| |
|
| Submission date |
Mar 16, 2015 |
| Last update date |
Dec 01, 2018 |
| Contact name |
Claus Juergen Scholz |
| Organization name |
University Hospital Würzburg
|
| Department |
Core Unit SysMed
|
| Street address |
Josef-Schneider-Str. 2
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE66937 |
The transcriptomes of suicide completers and deceased controls. |
|
