|
| Status |
Public on Mar 10, 2015 |
| Title |
U937_EVI1 cells, etoposide treatment, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
U937_EVI1 cells, 400 nM etoposide, 48 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U937
vector: EVI1 expression vector
treatment: 400 nM etoposide for 48h
|
| Treatment protocol |
Logarithmically growing cells were treated or not with 400 nM etoposide for 48 h
|
| Growth protocol |
RPMI1640, 10% FCS, 1% penicillin/streptomycin/glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini kit (Quiagen)
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Data processing |
RMA
|
| |
|
| Submission date |
Mar 09, 2015 |
| Last update date |
Mar 10, 2015 |
| Contact name |
Rotraud Wieser |
| E-mail(s) |
[email protected]
|
| Organization name |
Medical University of Vienna
|
| Department |
Clinic of Medicine I
|
| Street address |
Waehringer Guertel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE66660 |
Gene expression changes in U937 cells in response to ectopic expression of EVI1 and/or etoposide treatment |
|
