|
| Status |
Public on Dec 05, 2007 |
| Title |
Experiment:Dexamethasone induction of GR-REV transgenic Ler seedlings |
| Sample type |
RNA |
| |
|
| Source name |
Transgenic Ler seedling carrying GRREV transgene, 1 hour after mock treatment
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Transgenic Ler seedling carrying GRREV transgene. Transgene is a fusion of glucocorticoid receptor to Arabidopsis REVOLUTA protein. Expression is driven by 35S promoter. Seedlings were 10 days old.
|
| Treatment protocol |
Seedlings were treated with dexamethasone in ethanol to a final concentration of 0.1% ethanol and 50 micrograms per ml dexamethasone for one hour before tissue was harvested and quick frozen.
|
| Growth protocol |
Seedlings were grown in liquid culture, in 50 ml MS salts plus glucose in 250 ml flasks, 100 seeds per flask. Flasks were shaken gently on a rotator under fluorescent lights (24 hour, 22 degrees). Seeds were stratified at 4 degrees in water for 2 days prior to adding to culture medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 100 mg of tissue using a QIAGEN RNeasy plant miniprep kit.
|
| Label |
biotin
|
| Label protocol |
Twenty micrograms biotinylated cRNA was prepared from the purified total RNA using standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Part of experiment to identify immediate downstream targets of Arabidopsis REV HD-ZIPIII transcription factor.
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Feb 09, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
M. Kathryn Barton |
| E-mail(s) |
[email protected]
|
| Phone |
650 325 1521 x224
|
| Fax |
650 325 6857
|
| Organization name |
Carnegie Institution
|
| Department |
Dept. of Plant Biology
|
| Street address |
260 Panama Street
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94025 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE7003 |
Experiment to identify downstream targets of Arabidopsis REVOLUTA (HDZIPIII) transcription factor. |
|
| Relations |
| Reanalyzed by |
GSE119083 |
