|
| Status |
Public on Dec 23, 2018 |
| Title |
LPA4-KO eWAT, biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
epididymal white adipose tissue excised from LPA4-KO mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: lysophosphatidic acid receptor 4-deficient (Lpar4 -/Y)
gender: male
age: 12 weeks
tissue: epididymal white adipose tissue
|
| Treatment protocol |
Excised epididymal white adipose tissue sample wassnap frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the tissues and cells with RNeasy Lipid Tissue Mini Kit (Qiagen K.K., Tokyo, Japan), in accordance with the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
biotinylated aRNA was obtained from 100 ng of purified total RNA using a GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
K4_eWAT
|
| Data processing |
CEL files were quantified with the Factor Analysis for Robust Microarray Summarization (FARMS) method, using the statistical language R (R-2.14.0) and Bioconductor 2.9.
|
| |
|
| Submission date |
Feb 20, 2015 |
| Last update date |
Dec 24, 2018 |
| Contact name |
Yuji Nakai |
| Organization name |
Hirosaki University
|
| Department |
Institute of Regional Innovation
|
| Lab |
Section of Food Sciences
|
| Street address |
2-1-1, Yanagawa
|
| City |
Aomori |
| State/province |
Aomori |
| ZIP/Postal code |
038-0012 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE66130 |
Gene expression profiles in epididymal white adipose tissue of lysophosphatidic acid receptor 4-KO mice |
| GSE66132 |
Gene expression profiles in white adipose tissues of lysophosphatidic acid receptor 4-KO mice |
|
