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Sample GSM1614087 Query DataSets for GSM1614087
Status Public on Sep 08, 2016
Title H82_COMB
Sample type genomic
 
Source name Lung
Organism Homo sapiens
Characteristics individual: Diseased donor
cell line: SCLC cell line H82
cell line type: MYC amplified
treatment: COMB
Treatment protocol GCRA: Dexamethasone (1uM) + Retinoic Acid (1uM) 120hours; COMB: Dexamethasone (1uM) + Retinoic Acid (1uM) + Aza (0.2uM) + SAHA (0.2uM) 120 hours. shRNA targeting BRG1 (an ATPase of the SWI/SNF Chromatin Remodeling Complex).
Growth protocol Cells where growth on RPMI media with 10% FBS, 1% PS
Extracted molecule genomic DNA
Extraction protocol Total DNA where extrated using Trizol Reagent.
Label Cy3, Cy5
Label protocol Standard Illumina labelling protocol
 
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
Description Sample name: h82scCOMB
Cancer cell line
Data processing Genome Studio V2011.2
 
Submission date Feb 19, 2015
Last update date Sep 08, 2016
Contact name Manel Esteller
Organization name IDIBELL
Department PEBC
Lab Cancer Epigenetics
Street address Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
City L'Hospitalet de Llobregat
State/province Barcelona
ZIP/Postal code 08908
Country Spain
 
Platform ID GPL13534
Series (2)
GSE66087 Patterns of changes in DNA global methylation following the treatment with GC/RA and with GC/RA plus AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines
GSE66245 Treatment with GC/RA and combination with AZA/SAHA in MYC amplified and BRG1 mutant lung cancer cell lines

Data table header descriptions
ID_REF
VALUE Average Beta
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
cg00050873 0.134229910923544 0.618577099558951
cg00212031 0 0.988455804813154
cg00213748 0.217738431698642 0.202073280001151
cg00214611 0.275743935207523 0.521233350449371
cg00455876 0 0.0110188163414536
cg01707559 0.488207903742226 0
cg02004872 0.0390510885287386 0
cg02011394 0.19956388790156 0.285020125873059
cg02050847 0.139942317407511 0.809712678632229
cg02233190 0.130119372332837 0.00284254610247214
cg02494853 0.00735360721192817 0
cg02839557 0.218418657900291 0.309596926399848
cg02842889 0.219178935902011 0.632034034588155
cg03052502 0.198487326233463 0.374675355011049
cg03155755 0.224879136135458 0.238855084015473
cg03244189 0.060069148694703 0
cg03443143 0 0.436539868200546
cg03683899 0.17479568549643 0.181262961665105
cg03695421 0.231313988257493 0.00911980566142123
cg03706273 0.00497501147584736 0

Total number of rows: 485512

Table truncated, full table size 14789 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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