|
| Status |
Public on Jan 27, 2015 |
| Title |
S2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse lung scc cell line1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
cell line: S2
|
| Growth protocol |
Both cell lines were cultured in DMEM containing 10% FBS and pen/strep, in an incubator at 37°C with a mixture of air and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cultured cells were harvested after twice washes with 1×PBS. Total RNA was isolated from the cell lines using TRIZOL (Invitrogen) and sent out for microarray.
|
| Label |
biotin
|
| Label protocol |
Affymetrix Mouse Genome 430 2.0 Array
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Genome 430 2.0 Array
|
| Scan protocol |
Affymetrix Mouse Genome 430 2.0 Array
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Jan 26, 2015 |
| Last update date |
Jan 27, 2015 |
| Contact name |
zuoxiang xiao |
| E-mail(s) |
[email protected]
|
| Phone |
301 846 1632
|
| Organization name |
NCI
|
| Street address |
Bldg 567, Rm. 274, 1050 Boyles Street
|
| City |
Frederick |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE65291 |
Gene expression alteration by different cell lines derived from lung SCC of IKKα mutant mice |
|
