cultivar: X4 genotype: selfing line phenotype: normal
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from both control and cold-treated samples with Trizol Reagent (Invitrogen,http://www.invitrogen.com). The RNA quality was checked on a 1.2% agarose gel using an RNase-free electrophoresis system.
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Description
This sample is selfing line of Petunia hybrida. It is the first of three 2h cold treatment at 2°C biological replicates used in this experiment.
Data processing
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
