|
| Status |
Public on Apr 27, 2007 |
| Title |
Normospermic individual: Sample N3 |
| Sample type |
RNA |
| |
|
| Source name |
Normal Human Sperm
|
| Organism |
Homo sapiens |
| Characteristics |
Fertility Status: Fertile
|
| Treatment protocol |
None
|
| Growth protocol |
Healthy human donor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Semen was collected then treated with Somatic Cell Lysis Buffer (0.1% sodium dodecyl sulfate [SDS], 0.5% Triton X-100) on ice for 30 minutes. Lack of somatic cell contamination and sperm count were established by microscopic examination. RNA was isolated from sample using Trizol. Samples were then treated with RNase-free DNase I.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix U133plus2 GeneChips , staining and washing were carried out as described (Affymetrix, Santa Clara).
|
| Scan protocol |
Scanning procedures were carried out as described by Affymetrix, Santa Clara.
|
| Description |
RNA abundance profile from a normospermic semen sample
|
| Data processing |
The data were analyzed with DChip using invariant set normalization and PM-only MBEI model settings.
|
| |
|
| Submission date |
Jan 25, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Stephen A Krawetz |
| E-mail(s) |
[email protected]
|
| Organization name |
Wayne State University
|
| Street address |
275 East Hancock, CS Mott Center
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48207 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE6872 |
Spermatozoal RNA Profiles (U133 Plus 2.0 Array) |
| GSE6969 |
Spermatozoal RNA Profiles from Normally Fertile and Heterogeneously Teratozoospermic Subjects |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
