|
| Status |
Public on Jul 22, 2010 |
| Title |
PCB77 treated HepG2 cells at T0.5, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 cell line from ATCC at 8th generation after 30 minutes of PCB77 treatment.
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: Male
age: 15 years adolescent male
|
| Treatment protocol |
Treated with PCB77 for 30 minutes
|
| Growth protocol |
Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
Gene expression data from HepG2 cell lines after 30 minutes of PCB77 treatment.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jan 25, 2007 |
| Last update date |
Jul 22, 2010 |
| Contact name |
Sisir K. Dutta |
| E-mail(s) |
[email protected]
|
| Phone |
202-806-6942
|
| Fax |
202-806-5832
|
| Organization name |
Howard University
|
| Department |
Biology
|
| Lab |
Molecular Genetics
|
| Street address |
415 College Street, NW
|
| City |
Washington DC |
| State/province |
DC |
| ZIP/Postal code |
20059 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE6869 |
Expression data from human liver cell line induced by PCB77 |
| GSE6878 |
Expression data from human liver cells |
|
