|
| Status |
Public on Apr 02, 2015 |
| Title |
Lung CD11bhi CD14hi moDCs rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL mouse lungs CD11bhi CD14hi DCs
|
| Organism |
Mus musculus |
| Characteristics |
age: 8-12 wks
gender: male
tissue: lung
strain: C57BL/6J
cell population: CD11bhi CD14hi dendritic cells (DCs)
|
| Treatment protocol |
Mice were given 50ul PBS containing 100ng lipopolysaccharide and 100ug ovalbumin to airway. Sixteen hours later, lungs were removed, and dendritic cells were purified by flow cytometry (purity >93%).
|
| Growth protocol |
Eight to twelve weeks old C57BL/6 male mice were used. Twenty mice were used for each biological replicates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from purified cells using QIAGEN Rneasy kit according to manufactore's instruction.
|
| Label |
biotin
|
| Label protocol |
Fifty (50) ng of total RNA was amplified as directed in the WT-Ovation Pico RNA Amplification System protocol, and labeling with biotin following the Encore Biotin Module.
|
| |
|
| Hybridization protocol |
5 μg of amplified biotin-aRNAs were fragmented and hybridized to each array for 17.5 hours at 45°C in a rotating hybridization. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression data from C57BL mouse lungs CD11bhi CD14hi DCs
|
| Data processing |
Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) using the MAS5 algorithm to generate .CHP files.
|
| |
|
| Submission date |
Jan 12, 2015 |
| Last update date |
Apr 02, 2015 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE64896 |
Gene expression of distinct lung dendritic cell subsets |
|
