|
| Status |
Public on Apr 23, 2007 |
| Title |
BALB-RAG_OVA Replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
mouse whole lung
|
| Organism |
Mus musculus |
| Characteristics |
8 wk old BALB/c (or BALB-RAG) mice were sensitized to ovalbumin (or PBS)]on days 1 and 7, and challenged with aerosolized ovalbumin (or PBS) on days 15-21, and samples harvested on day 22
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using TRIZOL reagent (Gibco-BRL Life Technologies) according to the manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on rat RAE-230A microrray gene chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression from allergen stimulated mouse lung
|
| Data processing |
GCRMA
|
| |
|
| Submission date |
Jan 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Xin Lu |
| E-mail(s) |
[email protected]
|
| Phone |
847-937-7942
|
| Organization name |
Abbott Laboratories
|
| Department |
Cancer Research
|
| Lab |
Cancer Genomics
|
| Street address |
100 Abbott Park Rd
|
| City |
Abbott Park |
| State/province |
IL |
| ZIP/Postal code |
60064 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6858 |
Expression data from experimental murine asthma |
|
| Relations |
| Reanalyzed by |
GSE119085 |
