|
| Status |
Public on Jan 22, 2007 |
| Title |
Truman_1-6_Pst-DC3000(avrRpm1)-4hpi_Rep2_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Col-5
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Stock Code: N1688
Developmental stage: 3.90
Tissue: whole leaves from the inner rosette (systemic/ uninoculated leaves)
|
| Treatment protocol |
Leaves were inoculated with avirulent Pseudomonas syringae pv tomato DC3000(avrRpm1). DC3000(avrRpm1) cultures were grown overnight in 10 ml of Kings B solution (King et al., 1954) supplemented with antibiotics. Plants were inoculated with DC3000 containing the vector plasmid pVSP61 (Innes et al., 1993) carrying the avirulence gene avrRpm1. Antibiotics rifampicin(50 lg ml)1) and kanamycin (25 lg ml)1) were used for selection of the bacterium and plasmid, respectively. Overnight cultures were washed once in 10 mM MgCl2 and final cell densities were adjusted to OD600 0.2. Both sides of a leaf were infiltrated on their abaxial surface with a needleless 1ml syringe. At least 5 leaves per plant were inoculated and 16 plants were pooled to make this sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
standard NASC extraction protocol
|
| Label |
biotin
|
| Label protocol |
standard NASC labeling protocol
|
| |
|
| Hybridization protocol |
standard NASC hybridization protocol
|
| Scan protocol |
standard NASC scanning protocol
|
| Description |
Location: Growth Room
Growth substrate: A 4:1 mixture of Levingtons F2 compost (Fisons, Ipswich, UK) and vermiculite
stratification: 2 days in fridge at 4oCA
Growth protocol: Arabidopsis thaliana genotypes were sown in Levingtons F2 compost (Fisons, Ipswich, UK) and vernalized for 2 days at 4oC. Plants were grown under short day conditions in a controlled environment chamber (12 h light, 100?150 uE at 22oC day, 20oC night) for 5 weeks before use
|
| Data processing |
The data were analysed using Affymetrix Microarray Suite 5.0 (MAS5)
|
| |
|
| Submission date |
Jan 22, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE6831 |
Systemic response to avirulent bacterial infection |
|
| Relations |
| Reanalyzed by |
GSE119083 |
