|
| Status |
Public on Feb 27, 2007 |
| Title |
P_aeruginosa_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
bronchial epithelial cells BEAS-2B, P_aeruginosa stimulated
|
| Organism |
Homo sapiens |
| Characteristics |
bronchial epithelial cells BEAS-2B, P_aeruginosa stimulated, 4h, replicate 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using HighPureTM RNA kit (Roche) as recommended by the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Affymetrix one round amplification
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
|
| Scan protocol |
Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
|
| Description |
Human bronchial epithelial cells BEAS-2B were stimulated in vitro with 10E8/ml
UV-inactivated P. aeruginosa or S. aureus or with RSV (MOI 1) for 4 h.
h.
|
| Data processing |
Expression VALUES were generated using RMA (Robust Multi-Array Average expression measure) using R 2.4.0; Absolute Detection (ABS_CALL) was generated using the function mas5calls of the affy package for R.
|
| |
|
| Submission date |
Jan 19, 2007 |
| Last update date |
Feb 27, 2007 |
| Contact name |
Joerg Mages |
| E-mail(s) |
[email protected]
|
| Organization name |
Technical University Munich
|
| Department |
Institute of Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstrasse 30
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE6802 |
Gene expression analysis of bronchial epithelial cells stimulated by different airway pathogens. |
|
