|
| Status |
Public on Jan 23, 2007 |
| Title |
3T3L1_adipocyte_differentiation_confluent_ChipA |
| Sample type |
RNA |
| |
|
| Source name |
3T3-L1 fibroblast;MDI differentiation; confluent
|
| Organism |
Mus musculus |
| Characteristics |
3T3-L1 fibroblast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on the Mu11K GeneChip Murine Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using a Hewlett Packard confocal laser scanner
|
| Description |
all steps as per Expression Analysis Technical Manual, 2001, Affymetrix.
|
| Data processing |
The data were analyzed with GeneChip 3.3 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 18, 2007 |
| Last update date |
Jan 23, 2007 |
| Contact name |
Kevin Jon Cheung |
| Organization name |
The Rockefeller University
|
| Department |
Molecular Genetics
|
| Lab |
Jeffrey Friedman
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL75 |
| Series (2) |
| GSE6794 |
Expression data from 3T3-L1 adipogenesis |
| GSE6795 |
Expression data from C57BL6 tissues and 3T3-L1 fibroblasts |
|
