|
| Status |
Public on May 15, 2007 |
| Title |
HNN052 |
| Sample type |
RNA |
| |
|
| Source name |
Head and neck normal
|
| Organism |
Homo sapiens |
| Characteristics |
Case: Head and neck normal
Anatomical sites: tonsil
gender: Male
age: 47
Tumor Stage: -
|
| Treatment protocol |
Samples were fresh frozen in liquid nitrogen and collected with patients' consent under approval of the Institutional Review Boards.
|
| Growth protocol |
Sample was harvested without further growth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Enzo BioArray HighYield RNA Transcript Labeling Kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the scanner of the Affymetrix GeneChip Analysis System.
|
| Description |
Head and neck cancer
|
| Data processing |
The data were analyzed with the Bioconductor using Affymetrix package version 1.10.0. Probeset summary measures were computed by robust multiarray averaging applied to the combined set of 84 microarrays.
|
| |
|
| Submission date |
Jan 17, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paul Ahlquist |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Oncology
|
| Street address |
1525 Linden Dr.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6791 |
Gene Expression Profiles of HPV-Positive and -Negative Head/Neck and Cervical Cancers |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
