|
| Status |
Public on Jul 04, 2007 |
| Title |
intestine_PPARa_kockout_24hfasted_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
small_intestine_complete_24h_fasted
|
| Organism |
Mus musculus |
| Characteristics |
Strain: (129S4/SvJae) (PPARalpha knockout)
Gender: male
Age: 14 weeks
Tissue: small intestine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from small intestinal samples using TRIzol reagent (Invitrogen, Breda, the Netherlands) according to the manufacturer’s instructions. RNA was treated with DNAse and purified using the SV total RNA isolation system (Promega, Leiden, the Netherlands).
|
| Label |
biotin
|
| Label protocol |
Detailed methods for the labeling and subsequent hybridizations to the arrays are described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual, Revision 3, from Affymetrix (Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Detailed methods for the labeling and subsequent hybridizations to the arrays are described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual, Revision 3, from Affymetrix (Santa Clara, CA).
|
| Scan protocol |
Detailed methods for the labeling and subsequent hybridizations to the arrays are described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual, Revision 3, from Affymetrix (Santa Clara, CA).
|
| Description |
small intestine PPARa knockout mice, fasted for 24 hours
|
| Data processing |
GCRMA version 2.20, emperical Bayes
|
| |
|
| Submission date |
Jan 11, 2007 |
| Last update date |
Jul 04, 2007 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
[email protected]
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE6864 |
Gene expression regulation of transporters and phase I/II metabolic enzymes in murine small intestine during fasting |
|
