|
| Status |
Public on Dec 31, 2016 |
| Title |
IBS_12 |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs_IBS
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: IBS
tissue: blood
cell type: peripheral blood mononuclear cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were extracted from blood using standard protocols. RNA was isolated using the Qiagen RNeasy mini Kit (cat 74104). Column based.
|
| Label |
biotin
|
| Label protocol |
After Purification, single-stranded cDNA was fragmented and labeled using the GeneChip WT Terminal Labeling kit from Affymetrix (900671)
|
| |
|
| Hybridization protocol |
The fragmented single-stranded cDNA was combined with hybridization controls from Affymetrix and hybridized in the HTA 2.0 array overnight for 16-18 hours in the Affymetrix Genechip Hybridization Oven 645
|
| Scan protocol |
Arrays were stained and washed in the Affymetrix GeneChip Fluidics Station 450. Mircoarrays were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
Raw data was extracted and preprocessed using Expression Console from Affymetrix. CEL files were generated by the scanner.
|
| |
|
| Submission date |
Nov 17, 2014 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Chang Lin |
| Organization name |
University of California, Los Angeles
|
| Department |
Medicine
|
| Lab |
Center for Neurobiology of Stress
|
| Street address |
10833 Le Conte Avenue, CHS 47-122
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE63379 |
Genome-wide Expression Profiling in Irritable Bowel Syndrome |
|
