|
| Status |
Public on Mar 27, 2007 |
| Title |
Lung myd88 inf repA |
| Sample type |
RNA |
| |
|
| Source name |
lung_myd88_inf_repA
|
| Organism |
Mus musculus |
| Characteristics |
RNA: whole lung
strain: C57BL/6
genotyp: myd88 -/-
infected: yes
|
| Treatment protocol |
Mice were infected intranasally with 2.5 x 106 of C. pneumoniae in 30 µl of PBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were sacrificed by CO2 inhalation three days post infection with C. pneumoniae. The lungs were flushed with 10 ml of PBS applied through the right atrium of the heart to remove blood. Immediately thereafter, lungs were transferred to pre-cooled tubes placed in ethanol/dry ice bath and after homogenization, RNA was isolated using Tri-Reagent ® (T9424, Sigma) as described by the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Affymetrix one round amplification
|
| |
|
| Hybridization protocol |
Affymetrix recommendation
|
| Scan protocol |
Affymetrix recommendation
|
| Description |
lung rna from myd88 knockout mice, 3 days after infection with Chlamydia pneumoniae
|
| Data processing |
Expression VALUES were generated using RMA (Robust Multi-Array Average expression measure) using R 2.4.0; Absolute Detection (ABS_CALL) was generated using the function mas5calls of the affy package for R.
|
| |
|
| Submission date |
Jan 09, 2007 |
| Last update date |
Mar 27, 2007 |
| Contact name |
Joerg Mages |
| E-mail(s) |
[email protected]
|
| Organization name |
Technical University Munich
|
| Department |
Institute of Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstrasse 30
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE6688 |
MyD88-dependent changes in the pulmonary transcriptome after infection with Chlamydia pneumoniae |
|
