|
| Status |
Public on Jun 22, 2015 |
| Title |
ΔnigR in glucose, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Streptococcus mutans UA159
|
| Organism |
Streptococcus mutans UA159 |
| Characteristics |
culture: planktonic cells
genotype: ΔnigR
|
| Treatment protocol |
Cells were grown to the mid-log phase (OD600 = 0.65) and harvested by centrifugation.
|
| Growth protocol |
Cells were grown in chemically-defined medium supplemented with 0.5% glucose.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed using beat-beater. Extraction of total RNA was performed using Ambion RiboPure Bacteria kit according to the manufacturers protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3 μg of cDNA were hybridized for 16 hr at 47℃ on GeneChip S. mutans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using a laser scanner 7G (Affymetrix)
|
| Description |
Gene transcription data from planktonic culture OD600=0.65
|
| Data processing |
Data was analyzed with Expression and Transcription Analysis Console software from Affymetrix using default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 12, 2014 |
| Last update date |
Jun 22, 2015 |
| Contact name |
Dragana Ajdic |
| E-mail(s) |
[email protected]
|
| Phone |
305-243-0475
|
| Organization name |
University of Miami
|
| Department |
Dermatology
|
| Street address |
1600 NW 10th Ave, RMSB 2023
|
| City |
Miami |
| State/province |
Florid |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL4769 |
| Series (1) |
| GSE63213 |
Regulation of PTSBio by transcriptional repressor NigR |
|
